
组织样品检测
- 胸腺:探析T人体细胞分裂、发展;
- 腮腺结:T受损内部、B受损内部、DC受损内部解析;
- 骨髓:B内部成长理论探索,红内部成长理论探索,造血干内部理论探索;
- 肾组织化:定性分析炎性神经元侵润;
- 肺聚集:小鼠肺聚集中免役内部成分数据剖析,癌症或疾病小鼠肺聚集中免役内部侵及数据剖析;
- 肤质:组织细胞炎性浸润性阐述;
- 脑策划 :解析策划 中炎性受损细胞侵及情况报告。
工作应用案例一:胸腺T神经元成长发育
Population | Gating Step | ||||
Th Cells | Live single | mCD4+ | mCD8- | / | / |
Tc Cells | Live single | mCD4- | mCD8+ | / | / |
DP Cells | Live single | mCD4+ | mCD8+ | / | / |
DN Cells | Live single | mCD4- | mCD8- | / | / |
DN2 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25+ |
DN3 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25+ |
DN1 Cells | Live single | mCD4- | mCD8- | mCD44+ | mCD25- |
DN4 Cells | Live single | mCD4- | mCD8- | mCD44- | mCD25- |
实验设计典例二:肾脏免役组织研究分析(12色实施方案)
Population | Gating Step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophiles | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophiles | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
DC Cells | Live single | Not eosinophils | mCD11b+ | mCD11c+ | / |
T Cells | Live single | Not eosinophils | mCD3+ | mCD335- | / |
B Cells | Live single | Not eosinophils | mCD19+ | / | / |
NK Cells | Live single | Not eosinophils | mCD3- | mCD335+ | / |
Th Cells | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc Cells | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
测试实例三:脑聚集浸泡天然免疫癌细胞进行分析(10色方案设计)
Population | Gating step | ||||
Total Leukocytes | Live single | mCD45+ | / | / | / |
Neutrophiles | Live single | mCD45+ | mCD11b+ | Ly6G+ | / |
Monocytes | Live single | Not Neutrophiles | mCD11b+ | Ly6C hi | / |
Eosinophils | Live single | Not monocytes | mCD11b+ | SSC-H hi | / |
Macrophage | Live single | Not eosinophils | mCD11b+ | F4/80+ | / |
T cells | Live single | Not eosinophils | mCD3+ | mCD19- | / |
B cells | Live single | Not eosinophils | mCD19+ | mCD3- | / |
Th | Live single | Not eosinophils | mCD3+ | mCD4+ | mCD8- |
Tc | Live single | Not eosinophils | mCD3+ | mCD4- | mCD8+ |
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